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Format
Quick-DNA/RNA Viral 96 Kit
Highlights
- Quick recovery of viral DNA/RNA from plasma, serum and other samples.
- Omits the use of organic denaturants and proteases.
- High-quality viral DNA/RNA is ready for RT-PCR, sequencing, etc.
| Cat # | Name | Size | Price | Quantity |
|---|
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| R1200-125 | DNA/RNA Shield (2X Concentrate) | 125 ml | $255.60 | |
| W1001-4 | DNase/RNase-Free Water | 4 ml | $14.00 | |
| W1001-10 | DNase/RNase-Free Water | 10 ml | $22.10 | |
| D7020-1-100 | Viral DNA/RNA Buffer | 100 ml | $190.20 | |
| C2002 | Collection Plate | 2 Plates | $25.60 | |
| C2004 | Zymo-Spin I-96 Plate | 2 Plates | $165.70 | |
| C2007-4 | 96-Well Plate Cover Foil | 4 Foils | $10.30 | |
Description
Performance
Technical Specifications
| Equipment | Centrifuge with microplate carriers with height tolerance of 60 mm (2.36 inches) |
|---|---|
| Purity | High-quality nucleic acids are ready for Next-Gen sequencing, RT/qPCR, hybridization, etc. |
| Sample Source | Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, swab, and fecal samples |
| Size Range | 50 nt to ~200 kb |
| Yield | 5 µg DNA and 10 µg RNA |
Resources
Documents
FAQ
The purpose of beta-mercaptoethanol is to help with deproteination. It is not necessary if you are working with simple samples such as swabs. It is recommended if you are working with protein rich samples such as plasma, serum, blood, saliva, sputum, etc.
Yes, these samples are compatible.
Yes, this kit will co-purify some DNA.
Most downstream application methods for viral detection do not require DNase treatment during purification. If necessary, DNase treatment can be perform post-purification and additional components can be purchased separately (i.e., DNase I, DNA Digestion Buffer, RNA Prep Buffer and RNA Wash Buffer).
Yes, the change from clear to yellow is a result of oxidation and will not affect the performance of the buffer.
The Viral RNA Buffer is guaranteed for one year from the date of purchase even with the addition of beta-mercaptoethanol. Just be sure to close the bottle cap tightly to prevent evaporation.
In most cases, it could be because the samples contain high amounts of protein or cellular debris. To help prevent this in future preps, it’s best to add beta-mercaptoethanol to the Viral RNA buffer, and/or implement a Proteinase K digestion step.
For optimal results and detection of viral target, collect sample in DNA/RNA Shield and perform Proteinase K treatment. In addition, add beta-mercaptoethanol to the Viral RNA Buffer prior to purification, as well as perform all steps at room temperature and centrifugation speeds at 10,000-16,000 x g to ensure no buffer retention.
This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.
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