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Total RNA Sequencing
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Compatible with low input, FFPE samples, and any organism (mammals, plants, bacteria, and more)
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Validated solution for unbiased gene expression profiles
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Comprehensive publication-ready bioinformatics gene expression analysis report
User Friendly & Comprehensive Report
Publication-Ready Gene Expression Analysis at the Touch of a Button
![Image showing 4 pages of the total RNA-Seq report](https://files.zymoresearch.com/images/reports_4_page.png)
Full Analysis Includes:
- Sample Distance Matrix (PCA/heatmap)
- Differential gene expression
- Scatter plot
- MA plot
- Gene set enrichment
Unbiased Gene Expression Data with Probe-Free Technology
Workflow Certified Using RNA Standards of Known Composition
Probe-Free rRNA Depletion
Unbiased Libraries
(264 genes affected)
![Plot graph of Probe-Free rRNA depletion unbiased libraries](https://files.zymoresearch.com/images/lp_rna-seq_services_probe-free_rrna_depletion.png)
Probe-Based rRNA Removal
Biased Libraries
(3,603 genes affected)
![Plot graph of Probe-Based rRNA removal biased libraries](https://files.zymoresearch.com/images/lp_rna-seq_services_probe-based_rrna_removal.png)
Robust Workflow Compatible with Any Sample Type
![Graph of depletion comparison between human, mouse and e.coli](https://files.zymoresearch.com/images/lp_rna-seq_services_diverse_sample_types.png)
microRNA Sequencing
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Efficient microRNA capture and isolation
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Compatible with cfRNA from plasma and serum samples
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Comprehensive publication-ready microRNA expression data analysis
Efficient microRNA Capture from Biological Samples
![Graph of percent algined to hg38](https://files.zymoresearch.com/images/lp_rna-seq_services_efficient_smallrna_hg38.png)
![Graph of percent algined to miRBase](https://files.zymoresearch.com/images/lp_rna-seq_services_efficient_smallrna_mirbase.png)
Total RNA-Seq Technical Specifications
Sequencing Platform | NovaSeq 6000™ or NovaSeq X™ |
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Read Length | 2 x 150 bp, Unique Dual Indexing (UDI) |
Sequencing Depth | ~30 mln read pairs (can be customized) |
Accepted Sample Types | RNA, Cells, Tissue, FFPE Tissue Samples |
Library Prep | Zymo-Seq RiboFree Total RNA Library Kit (cat. R3000) |
rRNA Depletion | Enzymatic rRNA depletion compatible with any species |
Data Visualization | Sample similarity matrix, MDS plot, heatmap with top gene expression patterns, scatter plot, MA plot |
Statistical Analysis | Differential gene expression analysis with DESeq2 package, gene set entrichment analysis (if pathway annotation is available) |
Tell Us About Your RNA-Seq Service Project
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