![Graphic showing basic functionality of Direct-zol RNA Purification Kits](https://files.zymoresearch.com/images/lp-direct-zol_banner_desktop_v2.jpg)
Direct-zol RNA Purification Kits
RNA from TRIzol® in 7 Minutes
The Direct-zol RNA kits provide a streamlined method for the purification of high-quality RNA directly from samples in TRI Reagent® or similar.
The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary.
SelectScience® Gold Seal of Quality 2020 Winner!
SelectScience® Seals of Quality recognize products that have consistently received multiple positive reviews on SelectScience® and are designed to assist scientists in making important purchasing decisions.
![Image of Direct-zol RNA Kit with a SelectScience Gold Seal of Quality](https://files.zymoresearch.com/images/lp-direct-zol_selectscience_gold_seal_winner.png)
No Chloroform. No Precipitation. No Phase Separation!
Direct-zol: 7 minutes
![pipette dropper filling column with pink liquid](https://files.zymoresearch.com/images/dz.gif)
Conventional Method: 90+ minutes
![column tube with liquid inside](https://files.zymoresearch.com/images/alcoholprecip2.png)
Higher RNA Yields
![Bar Graph chart showing higher RNA yields from cells using Direct-zol compared to the conventional method.](https://files.zymoresearch.com/images/lp-direct-zol_higher_rna_yields_1.png)
![Bar Graph chart showing higher RNA yields from liver tissue using Direct-zol compared to the conventional method.](https://files.zymoresearch.com/images/lp-direct-zol_higher_rna_yields_2.png)
Direct-zol RNA kits recover ~4-fold more miRNAs(< 40 nt) than conventional methods.
Workflow and Compatibilities
![Image showing the workflow and compatibilities of Direct-Zol](https://files.zymoresearch.com/images/lp-direct-zol_workflow_and_compatibilities.jpg)
![Image showing the compatible input sample types, including plants, virus, bacteria, cells, and nasal swabs.](https://files.zymoresearch.com/images/lp-direct-zol_workflow_and_compatibilities-mobile_1.jpg)
![Image showing RNA being dispensed from a column filled with pink liquid.](https://files.zymoresearch.com/images/lp-direct-zol_workflow_and_compatibilities-mobile_2.jpg)
![Image showing the downstream applications of Direct-Zol, including Reverse Transcriptase PCR (RT-PCR) and Next-Gen Sequencing(NGS)](https://files.zymoresearch.com/images/lp-direct-zol_workflow_and_compatibilities-mobile_3.jpg)
The Only Automated Solution for TRIzol® Samples
High Quality, NGS-Ready RNA
![Image showing the amount of high quality, NGS ready RNA can be obtained from Trizol Samples](https://files.zymoresearch.com/images/lp-direct-zol_the_only_automated_solution.jpg)
The Direct-zol-96 MagBead RNA method eliminates traditional chloroform, phase separation, and preciptiation steps, enabling a fully automatable TRIzol® extraction. Just add ethanol to any TRIzol® sample, bind to magnetic beads, wash, and elute NGS-ready RNA.
Which Direct-zol RNA Kit is Right for You?
Trusted by Leading Institutions
Citations
- Sir Karakus, G., Tastan, C., Dilek Kancagi, D. et al. Preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates. Sci Rep 11, 5804 (2021). https://doi.org/10.1038/s41598-021-83930-6.
- Suntsova, M., Gaifullin, N., Allina, D. et al. Atlas of RNA sequencing profiles for normal human tissues. Sci Data 6, 36 (2019). https://doi.org/10.1038/s41597-019-0043-4.
- Ramos-Mandujano, G., Salunke, R., Mfarrej, S., Rachmadi, A. T., Hala, S., Xu, J., … Li, M. (2021). A Robust, Safe, and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Wastewater Samples. Global Challenges, 2000068. doi:10.1002/gch2.202000068.
- Christina Chuong, Tyler A. Bates, James Weger-Lucarelli, Infectious cDNA clones of two strains of Mayaro virus for studies on viral pathogenesis and vaccine development, Virology, Vol 535, 2019, Pages 227-231, ISSN 0042-6822, https://doi.org/10.1016/j.virol.2019.07.013.
- Christopher Smith, Nural Cokcetin, Thuyen Truong, Elizabeth Harry, Gyorgy Hutvagner, Sarah Bajan, Cataloguing the small RNA content of honey using next generation sequencing, Food Chemistry: Molecular Sciences, Volume 2, 2021, 100014, ISSN 2666-5662, https://doi.org/10.1016/j.fochms.2021.100014.
- Ren, Li-Li; Wang, et. al. Identification of a novel coronavirus causing severe pneumonia in human: a descriptive study, Chinese Medical Journal: May 5, 2020 - Volume 133 - Issue 9 - p 1015-1024 doi: 10.1097/CM9.0000000000000722.
- Patricia Angélica Barril, Luis Alfredo Pianciola, Melina Mazzeo, María Julia Ousset, María Virginia Jaureguiberry, Mauricio Alessandrello, Gloria Sánchez, Juan Martín Oteiza, Evaluation of viral concentration methods for SARS-CoV-2 recovery from wastewaters, Science of The Total Environment, Volume 756, 2021, 144105, ISSN 0048-9697, https://doi.org/10.1016/j.scitotenv.2020.144105.
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