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Format
Direct-zol-96 RNA Kits
Highlights
- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased: Complete RNA recovery without miRNA loss.
*For best performance with tissue samples, please select Miniprep Plus or MagBeads
| Cat # | Name | Size | Price | Quantity |
|---|
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| E1010-1-16 | DNA Digestion Buffer | 16 mL | $33.90 | |
| R2050-1-200 | TRI Reagent | 200 ml | $263.80 | |
| E1010-1-4 | DNA Digestion Buffer | 4 mL | $17.50 | |
| C2003 | Elution Plate | 2 Plates | $22.10 | |
| C2002 | Collection Plate | 2 Plates | $25.60 | |
| C2007-2 | 96-Well Plate Cover Foil | 2 Foils | $10.30 | |
| C2007-4 | 96-Well Plate Cover Foil | 4 Foils | $10.30 | |
| C2004 | Zymo-Spin I-96 Plate | 2 Plates | $165.70 | |
| W1001-30 | DNase/RNase-Free Water | 30 ml | $25.60 | |
| W1001-10 | DNase/RNase-Free Water | 10 ml | $22.10 | |
| R2050-2-160 | Direct-zol RNA PreWash (Concentrate) | 160 ml | $193.70 | |
| R2054 | Direct-zol-96 RNA | 2 x 96 preps | $487.80 | |
| R2056 | Direct-zol-96 RNA | 4 x 96 preps | $787.70 | |
| R1003-3-48 | RNA Wash Buffer | 48 ml | $122.60 | |
| E1010 | DNase I Set | 250 U | $63.40 | |
Description
Performance
Technical Specifications
| Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. |
|---|---|
| Equipment | Centrifuge with microplate carriers with height tolerance of 60 mm (2.36 inches) |
| Sample Inactivation | TRI Reagent® (provided with R2055, R2057) inhibits RNase activity and inactivates viruses and other infectious agents. |
| Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). |
| Size Range | Total RNA ≥ 17 nt |
| Yield | 10 µg RNA (binding capacity), ≥10 µl (elution volume) |
Resources
Documents
FAQ
All kit components are available for purchase separately.
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add an equal volume (1:1) of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.
Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)
Yes, proteins can be acetone precipitated from the column flowthrough. Please see the Protein Purification appendix in the protocol.
Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Direct-zol DNA/RNA (R2080) kits can isolate DNA from TRIzol.
Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.
The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.
Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
| Input | Average RNA Yield |
|---|---|
| Cells | 10 µg (per 106 cells) |
| HeLa | 15 µg |
| High Yield Tissue (mouse) | ≥ 30 µg (per 10 mg) |
| Spleen | 30-50 µg |
| Liver | 40-60 µg |
| Low Yield Tissue (mouse) | ≤ 30 µg (per 10 mg) |
| Brain, Heart | 5-15 µg |
| Muscle | 5-20 µg |
| Lung | 10-20 µg |
| Intestine | 10-30 µg |
| Kidney | 20-30 µg |
| Whole Blood | (per 1 ml) |
| Porcine | 10-20 µg |
| Human | 2-10 µg |
Purity, RIN and/or any type of contamination can result from initial sample preparation (i.e., inefficient lysis of the sample). To improve, increase the volume of the lysis reagent (e.g., TRI Reagent/TRIzol or RNA Lysis Buffer).
Yes, the Direct-zol RNA kits are compatible with CTAB-based RNA extraction methods for polysaccharide-rich and/or phenolics-rich samples (e.g., Pinus, Geranium plants). Please find detailed protocol here.
Reviews
"No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification."
Adina B.
University of Guelph
"This kit is amazing, I've got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!"
R.K.
CSU
"Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years."
H.Z.
Harvard Medical School
Citations
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