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    Direct-zol-96 MagBead RNA


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    Cat # Name Size Price Quantity
    R2103 Direct-zol-96 MagBead RNA (Product Supplied w/ 200 ml x 2 TRI Reagent) 4 x 96 preps $1,595.30
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    R2102 Direct-zol-96 MagBead RNA 4 x 96 preps $1,083.10
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    R2100 Direct-zol-96 MagBead RNA (96 preps) 1 x 96 preps $339.90
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    R2101 Direct-zol-96 MagBead RNA (Product Supplied w/ 50 ml x 2 TRI Reagent) 1 x 96 preps $503.10
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    Highlights

    • Easy Handling: Bypass chloroform, phase separation and precipitation steps.
    • NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
    • Non-Biased: Complete RNA recovery without miRNA loss.
    Description & Documents Specifications FAQ Components Citations

    Documents

    Supplemental Info

    Product Description

    The Direct-zol™-96 MagBead RNA kit provides a high-throughput (96-well), magnetic bead-based isolation of high-quality RNA directly from samples in TRI Reagent® and similar. The extraction method inactivates viruses and other infectious agents. Total RNA including small/microRNAs (≥17 nt) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRI Reagent®, wash and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including Next-Gen sequencing, RT-PCR, transcription profiling, hybridization, etc.

    Learn More

    For automation scripts and support, email automation@zymoresearch.com

    Documents

    Technical Specifications

    Compatibility TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
    Equipment Microcentrifuge, vortex, magstand
    Sample Inactivation TRI Reagent® (provided with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents.
    Sample Source Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
    Size Range Total RNA ≥ 17 nt
    Yield 10 µg RNA (binding capacity), ≥ 30 µl (elution volume)
    Supplemental Info

    Resources

    Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)

    All kit components are available for purchase separately.

    Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.

    If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

    Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add an equal volume (1:1) of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.

    Yes, proteins can be acetone precipitated from the column flowthrough. Please see the Protein Purification appendix in the protocol.

    Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.

    Direct-zol DNA/RNA (R2080) kits can isolate DNA from TRIzol

    Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.

    The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.

    Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.

    Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.

    Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.

    Input Average RNA Yield
    Cells 10 µg (per 106 cells)
    HeLa 15 µg
    High Yield Tissue (mouse) ≥ 30 µg (per 10 mg)
    Spleen 30-50 µg
    Liver 40-60 µg
    Low Yield Tissue (mouse) ≤ 30 µg (per 10 mg)
    Brain, Heart 5-15 µg
    Muscle 5-20 µg
    Lung 10-20 µg
    Intestine 10-30 µg
    Kidney 20-30 µg
    Whole Blood (per 1 ml)
    Porcine 10-20 µg
    Human 2-10 µg

    Purity, RIN and/or any type of contamination can result from initial sample preparation (i.e., inefficient lysis of the sample). To improve, increase the volume of the lysis reagent (e.g., TRI Reagent/TRIzol or RNA Lysis Buffer).

    • To ensure complete lysis, increase the volume of the lysis reagent (i.e., increase or titrate the volume of TRI Reagent/TRIzol or RNA Lysis Buffer). Lysate should be clear (not opaque or viscous). Pellet debris by centrifugation (if needed) and process the cleared supernatant.
    • Prior to adding TRI Reagent/TRIzol or RNA Lysis Buffer, perform enzymatic treatment (Proteinase K; #D3001-2-20) and/or mechanical homogenization (bead beating with ZR BashingBead Lysis Tubes; #S6003) in DNA/RNA Shield (#R1100).

    Cat # Name Size Price
    D4100-2-3
    D4100-2-12
    W1001-30 DNase/RNase-Free Water 30 ml $24.90
    R1060-2-100 RNA Prep Buffer 100 ml $138.20
    R1060-2-25 RNA Prep Buffer 25 ml $45.30
    R2130-1-120 MagBead DNA/RNA Wash 1 120 ml $224.30
    R2130-1-30 MagBead DNA/RNA Wash 1 30 ml $71.40
    R2130-2-20 MagBead DNA/RNA Wash 2 20 ml $61.20
    R2130-2-80 MagBead DNA/RNA Wash 2 80 ml $193.70
    C2002 Collection Plate 2 Plates $24.90
    C2003 Elution Plate 2 Plates $21.50
    C2007-8 96-Well Plate Cover Foil 8 Foils $18.50
    R2050-1-200 TRI Reagent 200 ml $256.10
    E1010-1-4 DNA Digestion Buffer 4 mL $17.00
    E1010 DNase I Set 250 U $63.40

    “Before I discovered this kit, I was isolating RNA the old school way with chloroform and it would take half the day to finish the protocol. The Direct-zol RNA Miniprep kit is AWESOME! It took hardly any time, the protocol was so easy, and my RNA quality was SO much better. Honestly, this kit revolutionized my life at the bench.”

    - A. Newhart (The Wistar Institute)

    “Simple protocol and yielded good quality of RNA. Only one kit working for all type of tissue, cell and especially biological fluids.”

    - Mohan K. (University of Illinois, Chicago)

    “Previously I used a protocol that took 3 hours, now I can have my RNA in 20 minutes. What is not to like about that? Just one column and two buffers, I love it.”

    - Arjan V. (Indiana University)

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