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OneStep PCR Inhibitor Removal Kit
![](http://www.zymoresearch.com/cdn/shop/files/D6031-OneStep-PCR-Inhibitor-Removal-Kit_large.webp?v=1724090569)
![](http://www.zymoresearch.com/cdn/shop/files/OneStep3tables_50dedee6-b22d-4d62-b9d9-31de727a3dae_large.webp?v=1724108652)
![](http://www.zymoresearch.com/cdn/shop/files/OneStepFilmGraph_large.webp?v=1724090569)
![](http://www.zymoresearch.com/cdn/shop/files/OneStepNGSDataChart_large.webp?v=1724090569)
![](http://www.zymoresearch.com/cdn/shop/files/OneStepMinimizeLossGraph_large.webp?v=1724090570)
![](http://www.zymoresearch.com/cdn/shop/files/D6031-OneStep-PCR-Inhibitor-Removal-Kit_medium.webp?v=1724090569)
![](http://www.zymoresearch.com/cdn/shop/files/OneStep3tables_50dedee6-b22d-4d62-b9d9-31de727a3dae_medium.webp?v=1724108652)
![](http://www.zymoresearch.com/cdn/shop/files/OneStepFilmGraph_medium.webp?v=1724090569)
![](http://www.zymoresearch.com/cdn/shop/files/OneStepNGSDataChart_medium.webp?v=1724090569)
![](http://www.zymoresearch.com/cdn/shop/files/OneStepMinimizeLossGraph_medium.webp?v=1724090570)
Cat # | Name | Size | Price | Quantity |
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Highlights
- Fast, one step procedure for cleaning impure samples prior to PCR, sequencing, NGS, reverse transcription (RT), etc.
- Efficiently eliminate polyphenolics from extracted DNA & RNA samples for PCR and short or long read sequencing.
- ≥80% recovery of PCR inhibitor free DNA & RNA.
Documents
Product Description
Technical Specifications
Applicable for | Cleaned samples are ready for PCR, sequencing, reverse transcription (RT), etc |
---|---|
Input Volume | 50-200µl |
Equipment | Microcentrifuge |
Purity | High quality, enzymatic-reaction ready DNA/RNA products. |
Sample source | Samples contaminated by polyphenolics that inhibit enzymatic reactions. |
Sample storage | Eluted DNA/RNA can be used immediately or stored at ≤ - 20°C. |
Type | ds/ssDNA and RNA. |
Yield | 80-90% |
Resources
Q1: What is the minimum and maximum input volume that can be added to the OneStep Inhibitor removal kit?
The minimum volume that can be processed with the HRC columns is 50µl. For the maximum volume, the columns can handle as much as 200µl, however, the input limit is mostly dependent on the amount of inhibitors present in the sample. For largely inhibited samples it is recommended to keep volumes between 50-100µl.
Q2: What is the difference between the old OneStep PCR Inhibitor Removal Kit (D6030) and this new version of the kit (D6031)?
The new version has a more simplified workflow as it does not require the addition of any prep buffer and has improved stability for more efficient inhibitor removal.
Q3: How do I access the old format?
The old format (D6030) will be available until July 2025. You can access the old format by clicking here.
Cat # | Name | Size | Price | |
---|---|---|---|---|
C1010-50 | Zymo-Spin IV-IR HRC Filters | 50 pack | $122.40 | |
C1001-50 | Collection Tubes | 50 Pack | $17.00 | |
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