Ultra-Fast
Simple 5 Minute Protocol
Inhibitor Free DNA & RNA
Completely Remove Polyphenolics
(i.e.
Hematin, Melanin, Bile Salts, etc.)
Highest Yield
Consistently Recover ≥ 80 % DNA & RNA
Achieve Inhibitor Free DNA & RNA in less than 5 minutes
Extract DNA & RNA From Sample:
Water
Plants
Soil
Remove Inhibitors in One Step:
Inhibitor Rich Eluate
Inhibitor Free DNA & RNA
Improve Downstream Analysis:
qPCR analysis of humic acid rich samples before and after OneStep™ Inhibitor Removal Kit. Humic acid rich samples were either untreated (brown) or treated (green) with OneStep™ PCR Inhibitor Removal Kit (D6031). Samples were then analyzed with Femto Human qPCR Premix (Zymo Research) and compared to DNA in pure water (red).
OneStep™ Treated DNA & RNA is Ideal for PCR & NGS
Successfully Amplify DNA From
Inhibitor-Rich Samples
Amplification of a 200 bp product from DNA containing humic acid is restored after treatment with the OneStep™ PCR Inhibitor Removal Kit.
Obtain NGS Data from Samples
High in Polyphenolics
Microbes are successfully detected in DNA extracted from soil using 16S sequencing following treatment with the OneStep™ PCR Inhibitor Removal Kit.
Minimize DNA & RNA Loss During Inhibitor Removal
Superior DNA Recovery
Comparison of DNA Recovery after PCR inhibitor removal using the OneStep™ PCR Inhibitor Removal Kit or Competitor Kits. 1kb ladder (Zymo Research) was diluted to various concentrations and treated with either the OneStep™ PCR Inhibitor Removal Kit or kits from Company Q or Company MN. DNA recovery was measured using Nanodrop (Thermo) or Qubit (Thermo) for lower concentrations.
The Market Leader in PCR Inhibitor Removal Technology
60% of all published articles that require PCR inhibitor removal choose OneStep™ PCR Inhibitor Removal Kit
Cited for Wastewater Surveillance, Pathogen Detection, Agriculture, Environmental Testing & Diagnostics in High Impact Journals
OneStep™ is the Clear Choice for Inhibitor Removal
| Zymo Research OneStep™ PCR Inhibitor Removal Kit* | Qiagen DNeasy or RNeasy Powerclean Pro** | Macherey-Nagel NucleoSpin Inhibitor Removal | |
|---|---|---|---|
| Time Per Prep | < 5 min | 10 min | 15 min |
| DNA & RNA Recovery | ≥ 80 % | ~ 55 % | 75 % |
| DNA,RNA, or Both | BOTH | Only DNA or only RNA | DNA |
| Maximum Sample Volume | Up to 200 µl | Up to 100 µl | Up to 100 µl |
| Max Nucleic Acid Input | No Limit | 20 µg | 60 µg |
| DNA Size Limit | No Limit | 60 - 50 kb | 200 bp - 50 kb |
| Elution Volume | N/A | > 50 µl | 50 - 100 µl |
- *OneStep™ PCR inhibitor removal kit filters do not bind to nucleic acid therefore do not have a binding capacity, max input, or elution volume.
- **Qiagen has one kit for DNA clean up and another kit for RNA clean up, OneStep™ PCR Inhibitor Removal kit can process either DNA or RNA with the same filters.
Well-Reviewed & Highly Trusted by Researchers
"Amazing, affordable, and essential...if you have samples with lots of secondary metabolites, like seaweeds, and the extraction kits used do not implement it already, you must get this."
- RK (University of Louisiana)
"PCR Inhibitor Removal Kit makes the impossible possible…I’m always impressed with its ability to transform the most difficult samples (those that are high in tannins and other inhibitors)."
- MD (Illinois Natural History Survey)
"Super quick and simple to use."
- BL (University of Vermont)
"Extremely useful to clean up extracts before PCR or library construction…a great aid when working with complex and generally “dirty” DNA extracts."
- MSV (Universada Nacional Autonoma de Mexico)
"An integral part of our eDNA survey assay…(the One Step PCR Inhibitor Removal Kit) has allowed for successful PCR amplification when the sample was previously inhibited."
- MO (Tangled Bank Conservation)
OneStep™ PCR Inhibitor Removal Technology
Simply add the OneStep™ PCR Inhibitor Removal Kit to your existing extraction workflow.
| Product | CAT No. | Prep Size |
|---|---|---|
| OneStep™ PCR Inhibitor Removal Kit | D6031 | 50 |
| D6035 | 2 x 96 |