The Definitive Gut Microbiome Standard

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True to Life Composition

21 Strains Mimic the Gut Microbiome

14%	Faecalibacterium prausnitzii
14%	Veillonella rogosae
14%	Roseburia hominis
14%	Bacteroides fragilis
6%	Prevotella copri
6%	Lactobacillus fermentum
6%	Bifidobacterium adolescentis
6%	Fusobacterium nucleatum
1.5%	Akkermansia muciniphila

Organisms in staggered abundances to represent predominant bacteria in the human gut

2.8%	Escherichia coli (strain 1)
2.8%	Escherichia coli (strain 2)
2.8%	Escherichia coli (strain 3)
2.8%	Escherichia coli (strain 4)
2.8%	Escherichia coli (strain 5)

5 strains of E. coli to challenge strain-level identification

1.5%	Clostridioides difficile
1.5%	Candida albicans
1.4%	Saccharomyces cerevisiae

0.1%	Methanobrevibacter smithii
0.01%	Salmonella enterica
0.001%	Enterococcus faecalis
0.0001%	Clostridium perfringens

Log abundance distribution to test sequencing detection limits

Strains representing predominant bacteria in the human gut

Strains often identified as pathogens

Eukaryote and Archaea cross-kingdom representation

Ahead of the Competition

ZymoBIOMICS Gut Microbiome Standard

Supplier A

Abundance Variation

< 15%

< 1,000%

Foreign Contaminants

< 0.01%

< 10%

Cross-kingdom Representation

Strict Manufacturing QC

Biosafety*

Non-Hazardous

BSL 2 Required

Cells per Tube†

2.96 x 10⁹

2.4 x 10⁸ ± 1 log

*The ZymoBIOMICS Gut Microbiome Standard is stored in DNA/RNA Shield, which inactivates all pathogens and allows for safe handling.

†Higher cell numbers more closely mimic the gut microbiome. This additionally allows for applications requiring large amounts of DNA, such as long-read sequencing.