Microbiome Standards

DNA from the ZymoBIOMICS Microbial Standard was extracted using four different methods: ZymoBIOMICS DNA Miniprep Kit (middle-left), HMP fecal DNA extraction protocol (middle), Supplier M (middle-right), and Supplier Q (right). Duplicates were performed. The extracted DNA was sequenced by targeted 16S rRNA gene sequencing. The theoretical/defined composition of the standard is shown on the left.

Microbial composition of ZymoBIOMICS standards determined by Shotgun Sequencing.
For the microbial standard (D6300), DNA was extracted using the ZymoBIOMICS DNA Miniprep kit. The library preparation was performed using Kapa HyperPlus kit. Sequencing was performed with Illumina HiSeq. Theoretical abundance (left) is given for comparison.

Foreign microbial DNA contamination assessed with deep shotgun sequencing.
In this test, 178 Million Illumina paired-end reads were generated. P. acnes (bottom) was the only foreign organism detected.

Log-Distributed Abundance

ZymoBIOMICS Microbial Community DNA Standard II (D6310) can assess both the detection limit and accuracy of your workflow over a broad-range (10²-10⁸ cells). This figure plots the composition of the standard as measured by NGS shotgun sequencing against the defined composition of the standard.

Test 16S Sequencing Workflows

ZymoBIOMICS Microbial Community DNA Standard II (D6311) was processed through 16S rRNA gene targeted sequencing and the sequencing data was analyzed using two bioinformatics pipelines, QIIME (red) and Dada2 (green). The theoretical abundance (blue) is displayed to facilitate comparison. The performance can be assessed regarding accuracy, false positive rates, and detection limit.