High-throughput: Magnetic bead-based clean-up of total RNA (including small/micro RNAs) from any enzymatic reactions and the aqueous phase following TRIzol® extractions.
Ultra-Pure: High-quality RNA ready for Next-Gen sequencing, RT-qPCR, hybridization, etc. DNase I included (R1082 only).
The RNA Clean & Concentrator Magbead kit provides a simple and reliable high-throughput method for the rapid preparation of high-quality, DNA-free (R1082) RNA. This simple procedure is based on the use of magnetic-bead based technology and a unique single-buffer system that allows for recovery of total RNA (> 17 nt) from any RNA sample. The procedure is easy: Add binding buffer and MagBinding Beads to your sample, then bind, wash and elute ultra-pure RNA. The highly concentrated, purified RNA is suitable for Next-Gen sequencing, RT-qPCR and other downstream applications.
Automated liquid handler or bead mover, plate shaker, and 96-well magnetic stand (not provided). The procedure can also be performed manually.
Purity
RNA is ready for Next-Gen Sequencing, RT-qPCR, microarray, hybridization, etc.
RNA Storage
RNA eluted with DNase/RNase-Free Water can be stored frozen. The addition of RNase inhibitors is optional but highly recommended for prolonged storage.
Sample Sources
DNase I treated RNA, aqueous phase following TRIzol/chloroform or similar extraction, in vitro transcription products, etc.
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