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    YeaStar RNA Kit

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    Zymo Research's 100% Satisfaction Guarantee

    Satisfaction Guaranteed

    Read The Zymo Research Promise

    Original Manufacturer. Made in USA.

    Cat # Name Size Price Quantity
    R1002 YeaStar RNA Kit 40 Preps $172.70
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    Highlights

    • Simple: Fast spin-column procedure yields pure yeast RNA without using glass beads or phenol.
    • Versatile: Efficient RNA isolation from a broad spectrum of fungal species susceptible to Zymolyase.
    • High-Quality: Isolated RNA is suitable for use in RT-PCR, Northern blotting, etc.
    Description & Documents Specifications FAQ Components Citations

    Documents


    Product Description


    The YeaStar RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans/, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Generally, the kit can be used for the purification of high-quality, total RNA from any fungus that can be lysed by yeast lytic enzyme. The kit facilitates the purification of 10-25 µg of total RNA from 1-1.5 ml of cultured cells using innovative Zymo-Spin column technology.

    Documents

    Technical Specifications

    Applicable For Isolated RNA is well suited for downstream applications such as Northern blotting, RT-PCR, Next-Generation Sequencing, Microarray, hybridization and other sensitive applications
    Elution Volume ≥ 60 µl DNase/RNase-Free Water
    Equipment Microcentrifuge & Heat Block/Bath
    Processing Time 30 minutes
    Processing Volume ≤ 1.5 ml of Culture (1-5 x 107 cells)
    Purity Typical A260/A280 & A260/A230 ≥ 1.8
    Sample Source Cell Culture (Colonies/Patches or Liquid Culture)
    Size Range ≥ 17 nt
    Type Total RNA
    Yield ≤ 25 µg of total RNA

    Resources


    Any strains susceptible to Zymolyase, which includes the following fungal genera: Asbya Kloekera Candida Kluyveromyces Debaryomyces Lipomyces Eremothecium Metschikowia Endomyces Pichia Hansenula Pullularia Hanseniaspora Saccharomyces Saccaromycodes Saccharomycopsis Schizosaccahromyces Torulopsis


    Cat # Name Size Price
    W1001-6 DNase/RNase-Free Water 6 ml $17.50
    R1001-1 YR Digestion Buffer 3.2 ml $26.90
    R1001-2 YR Lysis Buffer 6.4 ml $26.90
    R1003-3-6 RNA Wash Buffer 6 ml $21.00
    C1001-50 Collection Tubes 50 Pack $17.50
    C1006-50-G Zymo-Spin IIICG Columns 50 Pack $64.20
    E1004 Zymolyase 1000 U $87.60

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