Easy Processing: Included Deparaffinization Solution for simple paraffin removal from FFPE samples. No xylene necessary.
Improved Recovery: Optimized Proteinase K digestion and heat ensures maximum recovery of RNA from FFPE tissue samples.
High Quality: Non-crosslinked, DNA-free RNA are ready for all downstream applications including PCR, Next-Gen Sequencing, etc.
Description & Documents
Specifications
Components
Documents
Product Description
The Quick-RNA FFPE Kit provides a simple and reliable method for RNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples. The unique chemistries of this FFPE tissue RNA extraction kit have been optimized for maximum recovery of both large and small RNA species. Simply deparaffinize FFPE tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin column technology. The FFPE RNA extraction result is high-quality total RNA (including small RNAs 17-200 nt) that is DNA-free and is ready for RT-PCR, hybridization, sequencing, etc.
Documents
Technical Specifications
Equipment
Microcentrifuge, vortex, heat block/bath
Purity
RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source
FFPE Samples: Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 mm2
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