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Read The Zymo Research PromiseQuick-DNA HMW MagBead Kit
| Cat # | Name | Size | Price | Quantity |
|---|
Highlights
- High Molecular Weight DNA: Extract high molecular weight DNA up to 150 kb from any sample.
- Ultra-Pure: Highest DNA yield and purity equipped with RNA removal technology.
- Third-Generation Sequencing Ready: Optimized for long read sequencing (including Oxford Nanopore™ and PacBio SMRT™ sequencing).
Documents
Product Description
Technical Specifications
| DNA Purity | High quality DNA is ready for all sensitive downstream applications such as long read sequencing, PCR, endonuclease digestion, Southern blotting, genotyping, Next-Generation Sequencing, bisulfite conversion, etc. (A260/A230 ≥ 1.8). |
|---|---|
| DNA Size | Capable of recovering genomic and mitochondrial DNA sized fragments up to 150 kb. If present, plasmid, parasitic, microbial, and viral DNA will also be recovered. |
| DNA Yield | The DNA binding capacity is 10 µg per 50 µl MagBinding Beads used. |
| Elution Volume | 50 µl DNA Elution Buffer per 33 µl MagBinding Beads |
| Equipment | Magnetic rack, shaker and/or rotator, and microcentrifuge (optional) |
| Sample Types | Any cells, solid tissue, whole blood, saliva, biological fluids, buccal, swabs, samples stored in DNA/RNA Shield, etc. |
Resources
Q1: What is the typical length of DNA extracted using this kit?
DNA profiles generated using this kit range from 20kb – 200kb depending on sample type and handling.
Q2: Has this kit been validated with Nanopore or PacBio SMRT sequencing?
Yes, you can directly input DNA extracted using this kit into any long-read sequencing workflow.
Q3: I need more than 10 µg of HMW DNA for my downstream application. Is there any way I can increase my yield?
To increase the amount of DNA recovered, simply increase the amount of MagBinding Beads and DNA Elution Buffer used in the system (e.g. 100 µl MagBinding Beads eluted in 150 µl DNA elution buffer can bind up to 20-25 µg of HMW DNA).
Q4: Can this kit be used with microbial or environmental samples?
ZymoBIOMICS DNA Kits are recommended for processing microbial and environmental samples for long-read metagenomics sequencing.
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| D3004-2-200 | g-DNA Wash Buffer | 200 ml | $61.20 | |
| D3004-4-50 | DNA Elution Buffer | 50 ml | $36.30 | |
| D3004-5-50 | DNA Pre-Wash Buffer | 50 ml | $29.50 | |
| D3001-2-20 | Proteinase K w/ Storage Buffer Set | 20 mg | $49.90 | |
| D4100-2-6 | MagBinding Beads | 6 ml | $78.20 | |
| D4077-1-150 | Quick-DNA MagBinding Buffer | 150 ml | $99.70 | |
| D4081-3-25 | Biofluid & Solid Tissue Buffer | 25 mL | $43.10 | |
“The kit was very user friendly and easy to use. I was very satisfied with the results and the size fragments that the kit yielded.”
- D. K. (BASF)
“Great results in terms of yield and integrity of HMW DNA”
- M.P. (U of Louisville)
“The yield is good and the size of the extracted DNA is really high.”
- M. M. (UCLA)
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