Quick & Easy: Simple spin-column based isolation from up to 3 mL of plasma, serum, or other biological liquids. No phenol/chloroform or precipitation.
Highest Yields: Efficient isolation of cell-free DNA and RNA. Recover up to 515 times more microRNA.
Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, nCounter, etc.
Description & Documents
Specifications
Components
Documents
Product Description
The Quick-cfDNA/cfRNA Serum & Plasma Kit enables simple and efficient preparation of all circulating cell-free DNA and RNA (including protein-bound, exosomal, microRNA, and other small RNA) from serum, plasma, and other biological fluids, without using toxic phenol-chloroform or cumbersome protein precipitation and removal steps. The kit efficiently separates cell-free DNA and RNA, with option to co-elute. Cell-free nucleic acids scale linearly relative to the sample input volume up to 3 ml. Zymo-Spin™ Technology enables isolation of ultra-pure cell-free nucleic acids suitable for all subsequent analyses, such as RT-qPCR and NGS.
Documents
Technical Specifications
Elution Volume
cfDNA and cfRNA can be eluted into 15 µl (to maximize total yield) or as little as 6 µl (for highly concentrated eluate) of DNase/RNase-Free Water.
Processing Time
Typically requires 30 minutes (cfDNA/cfRNA co-elution protocol) to 45 minutes (cfDNA/cfRNA separation protocol) hands-on time to process 10 samples. Sample digestion requires 2 hours.
Purity
High quality cell-free DNA (cfDNA) and cell-free RNA (cfRNA) are ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing.
Required Equipment
Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold.
Sample Types
Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids.
Size
DNA ≥ 50 bp and RNA ≥ 17 nt.
Yield
Yields are sample dependent and may vary. Typical recovery ranges from 1 – 100 ng/ml of human plasma or serum for both cfDNA and cfRNA. Kit is optimized for recovery of total cfDNA and cfRNA, including small RNA and microRNA.
Resources
Cat #
Name
Size
Price
R1072-1-150
Quick-cfDNA/cfRNA Digestion Buffer
150 mL
$305.90
R1072-2-150
Quick-cfDNA/cfRNA Binding Buffer
150 mL
$305.90
W1001-10
DNase/RNase-Free Water
10 ml
$21.50
R1003-3-12
RNA Wash Buffer
12 ml
$34.00
R1060-2-100
RNA Prep Buffer
100 ml
$138.20
R1072-3-20
Cell-free Recovery Buffer
20 ml
$51.00
C1004-50
Zymo-Spin IC Columns
50 Pack
$60.00
C1006-50-F
Spin-Away Filters
50 Pack
$62.30
C1039-25
25 ml Reservoir for Spin-Away Filters and Zymo-Spin IIICG
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