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    Proteinase K w/ Storage Buffer Set


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    Cat # Name Size Price Quantity
    D3001-2-5 Proteinase K w/ Storage Buffer Set 5 mg $23.80
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    D3001-2-20 Proteinase K w/ Storage Buffer Set 20 mg $49.90
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    D3001-2-60 Proteinase K w/ Storage Buffer Set 60 mg $149.60
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    D3001-2-125 Proteinase K w/ Storage Buffer Set 125 mg $257.20
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    Highlights

    • Lyophilized enzyme provided with Proteinase K Storage Buffer.
    Description & Documents Specifications FAQ

    Documents

    Supplemental Info

    Product Description

    Proteinase K is a stable serine protease with broad substrate specificity and will degrade many proteins in their native conformation even in the presence of detergents (e.g., SDS). The enzyme is frequently used in molecular biology applications to digest unwanted proteins such as nucleases from DNA and/or RNA preparations from microorganisms, cells, and plants.

    Documents

    Technical Specifications

    Enzyme Commission Number (EC 3.4.21.64)
    pH and Temperature Range 4.0 to 12.0 (8.0 is optimum), 25 to 65°C
    Source Engyodontium album
    Specific Activity > 30 units/mg protein
    Unit Definition One unit (U) of enzyme will hydrolyze urea-denatured hemoglobin to produce 1.0 µmole of tyrosine per minute at pH 7.5 at 37°C.
    Supplemental Info

    Resources

    Size Add Proteinase K Storage Buffer
    5 mg 260 µl
    20 mg 1040 µl
    60 mg 3120 µl
    125 mg 6500 µl
    Reconstitute lyophilized Proteinase K at 20 mg/ml with Proteinase K Storage Buffer. Table provides recommended volumes for different sample sizes (mg); Concentration can be adjusted as needed.

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