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    His-Spin Protein Miniprep


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    Cat # Name Size Price Quantity
    P2001 His-Spin Protein Miniprep 10 Preps $83.80
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    P2002 His-Spin Protein Miniprep 50 Preps $329.70
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    Highlights

    • Fast: Purify His-tagged proteins from cell lysates in less than 5 minutes.
    • Simple: Prepare pure protein for small-scale studies using a spin-column.
    • Convenient: No special instrumentation needed other than a benchtop microcentrifuge.
    Description & Documents Specifications FAQ Components

    Documents

    Product Description

    The His-Spin Protein Miniprep provides a fast spin-column based purification technology for His-tagged proteins. Up to 1 mg of His-tagged protein can be purified in 5 minutes and eluted in as little as 100 µl of His-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.

    Documents

    Technical Specifications

    Affinity Matrix Nickel charged agarose
    Binding Capacity 1 mg
    Elution Method Imidazole gradient
    Elution Volume 100 - 200 µl (150 µl is optimal)
    Principle of Technology Based on a nickel-charged His-Affinity Gel (IMAC).
    Processing Time 5 minutes
    Product Storage Please store the His-Affinity Gel, His-Binding Buffer, His-Wash Buffer, and His-Elution Buffer at 4°C. The other components can be stored at room temperature.
    Protein Purity Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications.
    Required Equipment Microcentrifuge
    Sample Type Cell lysates or other complex protein mixtures containing His-tagged proteins.

    Resources

    Yes, the His-Spin Protein Miniprep Kit can also be used with eukaryotic expression systems. For references: Marcos J. Caballero-L´opez, Manuel Nieto-D´ıaz, M´onica Yunta, David Reigada, Teresa Mu˜noz-Galdeano, ´Angela del ´Aguila, Rosa Navarro-Ru´ız, Wolfang Pita-Thomas, Dan Lindholm, Rodrigo M. Maza - XIAP Interacts with and Regulates the Activity of FAF1 – 2017, BBA - Molecular Cell Research. doi:10.1016/j.bbamcr.2017.04.006

    Smaller elution volumes are possible and may yield more concentrated protein, but the elution efficiency may be compromised.

    - The His-tag may be rendered inaccessible due to protein folding. The protein can be purified at denaturing conditions. - The recombinant protein can become insoluble as a result of overexpression. The protein can be purified at denaturing conditions. - Starting material is too dilute. If the starting material contains very low levels of His-tagged protein, then it may require more than 300 µl of sample volume to purify enough protein. Simply repeat steps 3 and 4 until the desired volume has been loaded onto the column. - The protein is bound to the His-Affinity Gel too tightly and can not be eluted with the supplied His-Elution Buffer. A custom-made elution buffer containing 500 mM imidazole or 100 mM EDTA may elute the tightly-bound protein. - Depending on the protein, lowering the imidazole concentration of the His-Wash Buffer to 25 mM (e.g., by dilution with His-Binding Buffer) may increase yields.

    EDTA, EGTA, DTT, > 15 mM Β-mercaptoethanol, and > 10 mM imidazole or histidine. If your starting material contains these compounds, dilution with the His-Binding Buffer may help. Multiple loadings on the column will be necessary to load enough material. If the sample is in a different buffer, adjust the pH and imidazole and salt concentrations and carry out a test preparation. If the protein is still not bound, the sample needs to be dialyzed before use.

    - Optimize expression conditions. Overexpression of proteins may result in formation of insoluble inclusion bodies inside cells. If a large band of overexpressed protein is visible after SDS-PAGE electrophoresis of whole cells, but the band is absent after SDS-PAGE electrophoresis of cleared cell lysates, this indicates that the protein may not be soluble and the expressed protein may form inclusion bodies. - Use Denaturing conditions. Insoluble proteins will not be purified using the provided buffers. It is, however, possible to purify such proteins at denaturing conditions in the presence of ≤ 8 M urea or ≤ 6 M guanidine hydrochloride. The protein native structure and thus enzyme activity is lost under such conditions, but may be restored by refolding the protein after purification.

    Membrane proteins can be purified after solubilization in a nonionic detergent. Concentrations of up to 2% of Triton® or TWEEN® can be present in the loaded sample.

    The pH value of the loaded sample should be between 7.5 and 8.0. Too high or low pH can result in decreased protein yields and/or quality.

    - Check your buffers for signs of contamination and check the pH of the buffers. - Increase centrifugation time and speed. Ensure that the His-Affinity Gel drains completely after each spin (some older centrifuge models may require a longer centrifugation time). - If the problem persists, additional wash steps can be added to the purification protocol, or increasing the imidazole concentration of the washing buffer to 60 – 100 mM (e.g., by dilution with the His-Elution Buffer).

    Is there a recommended lysis protocol? For protein purification from E. coli lysates: 1. Harvest & pellet 10 ml of E. coli culture. 2. Resuspend in 1 ml of His-Binding Buffer. 3. Lyse the cells by sonication (or other methods). 4. Spin at ≥ 12,000 x g at 4°C for 5 minutes. 5. Use 150 µl of the supernatant for the His-Spin Protein Miniprep protocol.

    Reducing Agents ≤ 15 mM β-mercaptoethanol Non-Ionic Detergents ≤ 2% Nonidet® P-40 ≤ 2% Triton® X-100 ≤ 2% Tween®-20 Denaturing Reagents ≤ 8 M Urea Chemicals and other Reagents ≤ 10 mM Imidazole ≤ 10 mM Histidine

    Cat # Name Size Price
    P2003-2 His-Affinity Gel 14 ml $215.30
    P2003-3 His-Binding Buffer 50 ml $28.30
    P2003-4 His-Wash Buffer 50 ml $30.60
    P2003-5 His-Elution Buffer 25 ml $36.30
    C1001-50 Collection Tubes 50 Pack $17.00
    P2003-1 Zymo-Spin P1 Columns 50 Pack $49.90

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