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Documents
Product Description
Technical Specifications
| Applicable For | Next-Generation Sequencing, qPCR, microarray |
|---|---|
| Device Specs | 20 x 76 mm screw cap tube prefilled with DNA/RNA Shield (9mL). Spoon attached to screwcap. |
| Device Storage | 5-30°C, 24 months |
| Registration Status | CE-IVD certified |
| Sample Collection | 1g or 1mL stool sample |
| Sample Source | Stool, soil, environmental samples |
| Stability | RNA: Ambient temperature (4°C- 25°C) at least 1 month DNA: Ambient temperature (4°C- 25°C) at least 2 years DNA & RNA: Frozen (< -20°C): Indefinitely |
Resources
Q1: Can DNA/RNA Shield be used on extracted DNA and/or RNA?
Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).
Q2: Can I still use DNA/RNA Shield if a precipitate has formed?
This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.
Q3: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
No, tissues do not need to be homogenized.
Q4: How to collect solid tissues in DNA/RNA Shield?
Transfer tissues in to DNA RNA Shield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.
Q5: Can I FACS sort directly into DNA/RNA Shield?
Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 µl of FACS sorted cells into minimally 400 µl of DNA/RNA Shield).
Q6: How long can samples be stored frozen in DNA/RNA Shield?
DNA & RNA (<-20°C): Indefinitely
Q7: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
RNA > 1 month
DNA > 2 years
Q8: What sample types is DNA/RNA Shield suitable for?
DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).
Q9: Is DNA/RNA ShieldTM compatible with bleach (sodium hypochlorite)?
Avoid mixing the reagent and bleach (sodium hypochlorite) or other strong oxidizing agents and acids. Clean up any accidental spills with water first.
“We extracted RNA from cells suspended in DNA/RNA Shield with the Direct-zol miniprep set from Zymo (using Trizol). It worked perfect for us.”
- Mariska B. (University of Amsterdam)
“I did an assay using RNA/DNA shield and it works perfect. The product have excellent reproducibility when stored at 4°C for up to a month.”
- Maha AE. (University of Florida)
“We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4°C or -20°C for months, never saw any DNA degradation.”
- Umberto P. (University of Pavia)
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