Low-input RRBS DNA Methylation Profiling
From as Little as 10ng of DNA
![cat, dog and child looking off to the side](https://files.zymoresearch.com/images/rrbs_lp_banner_hero.png)
Reduced Representation Bisulfite Sequencing (RRBS) Is Informative and Efficient
Needs only ~ 10% of sequencing reads compared to whole-genome bisulfite sequencing
Covers ≥70% of promoters, CpG islands, gene bodies, and ~ 35% of enhancers*
Ideal for high throughput DNA methylation screening at single nucleotide resolution
RRBS Is Applicable Across Diverse Species
![Clipboard with sheet of paper. Human, Mouse, Chicken and Platypus are the options and they are checked off](https://files.zymoresearch.com/images/rrbs_lp_clipboard.png)
RRBS Has Many Popular Applications
Developmental Biology
![Clipart image of embryo](https://files.zymoresearch.com/images/rrbs_lp_baby.png)
Cancer Research
![Clipart image of cancer cells](https://files.zymoresearch.com/images/rrbs_lp_cancer.png)
Drug Response Study
![Clipart image of pills with bottle](https://files.zymoresearch.com/images/rrbs_lp_pills.png)
Biomarker Discovery
![Clipart image of dna strand with magnifying glass](https://files.zymoresearch.com/images/rrbs_lp_biomarker.png)
Zymo-Seq RRBS Provides A Simple Solution for NGS Library Preparation
The workflow minimizes hands-on time.
![Flow chart showing the process of hands-on time](https://files.zymoresearch.com/images/rrbs_lp_wokflow.png?v=2)
Zymo-Seq RRBS Detects a Significant Number of CpG Sites
![Chart showing the number of CpGs across the genome](https://files.zymoresearch.com/images/rrbs_lp_methy_ip.png)
Distribution of CpG sites detected (≥ 5X, green dots) in a library constructed with Zymo-Seq RRBS Library Kit across the human chromosomes using 10 ng of human liver genomic DNA
Zymo-Seq RRBS Delivers Reproducible Results Regardless of Input Amount
CpG Sites Overlap
![Venn Diagram of CpG Sites Overlap](https://files.zymoresearch.com/images/rrbs_lp_reproducible_cpg.png)
Methylation Ratio Correlation
![Plot Map of Methylation Ration Correlation](https://files.zymoresearch.com/images/rrbs_lp_methylation_ratio.png)
Libraries constructed with Zymo-Seq RRBS Library Kit using 10 ng and 100 ng of human liver genomic DNA.
Left: Overlap of CpG sites detected (≥5X). Right: Methylation ratio correlation of CpG sites detected (≥50X)
Simplify Your Research With The Zymo-Seq RRBS Library Kit
![The Zymo-Seq RRBS Library Kit Product Image](https://files.zymoresearch.com/product-images/d5460_ez_dna_methylation_rrbs_library_prep_kit.png)
* Enhancers derived from H3K4me2 peaks. Data based on libraries generated using Zymo-Seq RRBS Library Kit with various amount of human liver genomic DNA.