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Efficient RNA Removal in Plasmid Purification
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Plasmid Purified using a plasmid miniprep with or without PureRec RNase A added to
resuspension buffer (P1 buffer). When PureRec RNase A is added, there is no RNA contamination in
the purified plasmid DNA. M: ZR 1 kb DNA marker (M5003-200). (-) No RNase A added to miniprep P1
buffer. (+) 100 μg/ml PureRec RNase A was added to P1 buffer.
Most other commercial RNase A is typically purified from bovine pancreas. While this source has been a traditional means of obtaining RNase A, it introduces potential risks of contamination.
PureRec RNase A is overexpressed, and extensively purified from a prokaryotic host. It is exceptionally stable and works in a wide range of buffer conditions and temperatures. PureRec RNase A is extremely active, free of DNase and guaranteed animal-free.