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    Zymoclean Large Fragment DNA Recovery


    Zymo Research's 100% Satisfaction Guarantee

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    Read The Zymo Research Promise

    Cat # Name Size Price Quantity
    D4045 Zymoclean Large Fragment DNA Recovery 25 Preps $113.30
    - +
    D4046 Zymoclean Large Fragment DNA Recovery 100 Preps $364.80
    - +

    Highlights

    • Quick (15 minute) recovery of large-sized DNA (e.g., genomic, plasmid [BAC/PAC], viral, phage, etc.) from agarose gels.
    • Unique column design for low volume (≥ 10 µl) elution of ultra-pure, high-yield DNA.
    • Eluted DNA is well-suited for use in endonuclease digestion, sequencing, labeling, PCR, etc.
    Description & Documents Specifications Components

    Documents

    Supplemental Info

    Product Description

    The Zymoclean Large Fragment DNA Recovery Kit is a gel DNA extraction kit that provides a streamlined method for the rapid purification and concentration of high-quality large-sized DNA from agarose gels. Simply add the specially formulated Agarose Dissolving Buffer (ADB) to the gel slice containing a DNA sample, let dissolve, and then transfer to the supplied Zymo-Spin IC-XL Column. There is no need for organic denaturants or chloroform. Instead, the product utilizes unique spin column technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean Large Fragment DNA Recovery Kit is ideal for PCR, sequencing, endonuclease digestion, ligation, etc. The entire procedure typically takes about 15 minutes.

    Documents

    Technical Specifications

    Applicable For High-quality, purified DNA is ideal for sequencing ligation reactions, and any other sensitive downstream applications
    Elution Volume ≥ 10 µl
    Equipment Microcentrifuge
    Purity High-quality, purified DNA is especially well-suited for sequencing and ligation reactions
    Sample Source Agarose gels containing large DNA fragments of interest
    Sample storage Eluted DNA can be used immediately or stored at ≤ -20°C
    Size Range 50 bp to 200 kb
    Type Large DNA
    Yield Up to 10 µg DNA
    Supplemental Info

    Resources

    Cat # Name Size Price
    C1001-50 Collection Tubes 50 Pack $17.00
    D3004-4-4 DNA Elution Buffer 4 ml $11.30
    D3004-4-1 DNA Elution Buffer 1 ml $12.50
    D4003-2-24 DNA Wash Buffer (Concentrate) 24 ml $37.40
    D4001-1-50 ADB (Agarose Dissolving Buffer) 50 ml $37.40
    D4001-1-100 ADB (Agarose Dissolving Buffer) 100 ml $71.40
    D4003-2-6 DNA Wash Buffer (Concentrate) 6 ml $11.30
    C1002-50 Zymo-Spin IC-XL 50 Pack $74.80
    C1002-25 Zymo-Spin IC-XL 25 Pack $38.50

    “The best advantage about this kit is that it is really quick to make it, and the total DNA recuperated have a good quality and it have a good concentration to follow the experiments.”

    - Ingrid M.

    “The ADB buffer and higher temperature used melted the gel quicker than previously used kits thus making DNA recovery using this kit more efficient and generally quicker than previously used kits.”

    - Kelly-Anne F.

    “The kit was very easy to follow and yielded good results. It was more affordable than the Qiagen Gel Extraction kits, but worked just as efficiently. Solid product“

    - Thomas B (University of Tennessee, Knoxville)

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