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Femto Fungal DNA Quantification Kit
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Cat # | Name | Type | Price | Quantity |
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Highlights
- Quantify as little 20 femtograms of fungal DNA in as little as 1 µl of sample.
- High specificity and sensitivity for fungal DNA in a background of non-target DNA.
- Fast and simple: add samples to the PreMix... and quantify.
Documents
Product Description
Technical Specifications
Detection Range | 20 fg to 20 ng DNA |
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Equipment | Real-time quantitative PCR system, vortex mixer, microcentrifuge, PCR tube strip or PCR plate; optically transparent sealing film for PCR plate or tube strip caps |
Sample Source | Purified DNA |
Resources
Q1: Is there any general advice on GLP for qPCR?
Since this kit is so sensitive, avoiding possible sources of contamination is key for a successful qPCR. - Work in a clean environment and decontaminate the benchtop and pipettes with a 10% bleach solution. - Wipe down the areas with ethanol to remove the trace amounts of bleach and allow the area to try. - Use filtered tips. - Take care to ensure that pipetting is efficient and accurate. Calibrate your pipettes regularly. - Add standards directly into the premix and pipet up and down to flush out any remaining liquid from the tip. - Avoid pipetting onto the walls as this may skew the results. - The SYTO9 dye present within the premix is sensitive to light, so working quickly and diligently is recommended.
Q2: Does qPCR Premix contain ROX?
Our qPCR Premixes does not contain ROX Reference Dye. Ensure that the ROX channel of your device is switched off.
Q3: Which settings can be used for signal detection?
Our qPCR Premixes contains Syto9. You can use the SYBR Green channel for signal detection.
Q4: Are the Femto kits certified for low bioburden?
No, kits are not certified.
Q5: What primers are used in E2007?
Primers are ITS1-F and ITS2-R. Forward Primer: ITS-1F 5'- CTTGGTCATTTAGAGGAAGTAA-3' Reverse Primer: ITS-2R 5'- GCTGCGTTCTTCATCGATGC-3' They are based on this publication: White, Thomas J., et al. "Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics." PCR protocols: a guide to methods and applications 18 (1990): 315-322
Q6: What is the primer target region?
The kit targets the ITS region
Q7: What is the origin of the fungal DNA Standards?
The DNA is derived from S. cerevisiae strain TMY18.
Cat # | Name | Size | Price | |
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E2007-1 | Femto Fungal qPCR Premix | Bacterial DNA | $211.90 | |
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