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Read The Zymo Research PromiseQuick-cfRNA Serum & Plasma Kit
| Cat # | Name | Size | Price | Quantity |
|---|
Highlights
- Quick & Easy: Simple spin-column based isolation from up to 3 mL of plasma, serum, or other biological liquids. No phenol/chloroform or precipitation.
- Highest Yields: Efficient isolation of total cell-free RNA. Recover up to 515 times more microRNA.
- Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, nCounter, etc.
Documents
Product Description
Technical Specifications
| Elution Volume | cfRNA can be eluted into 15 µl (to maximize total yield) or as little as 6 µl (for highly concentrated eluate) of DNase/RNase-Free Water. |
|---|---|
| Processing Time | Typically requires 30 minutes hands-on time to process 10 samples. Sample digestion requires 2 hours. |
| Purity | High quality cell-free RNA (cfRNA) is ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing. |
| Required Equipment | Vacuum manifold, microcentrifuge, vortex, water bath or heating block, and vacuum. |
| Sample Type | Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids. |
| Size | RNA ≥ 17 nt. |
| Yield | Yields are sample dependent and may vary. Typical recovery ranges from 1 – 100 ng/ml of human plasma or serum. Kit is optimized for recovery of total cfRNA, including small RNA and microRNA. |
Resources
Q1: Can saliva be used as sample input?
Saliva must be cleared to remove debris/mucus (12,000xg for 15 min at room temperature). Up to 1 ml cleared salvia can be used per extraction.
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| R1059-4-100 | Quick-cfRNA Binding Buffer | 100 ml | $143.90 | |
| R1059-3-50 | Quick-cfRNA Digestion Buffer | 50 ml | $130.80 | |
| R1003-3-12 | RNA Wash Buffer | 12 ml | $34.00 | |
| R1070-1-10 | RNA Recovery Buffer | 10 ml | $20.40 | |
| R1060-2-50 | RNA Prep Buffer | 50 ml | $69.10 | |
| C1001-50 | Collection Tubes | 50 Pack | $17.00 | |
| C1004-50 | Zymo-Spin IC Columns | 50 Pack | $60.00 | |
| C1039-25 | 25 ml Reservoir for Spin-Away Filters and Zymo-Spin IIICG | 25 Pack | $46.50 | |
| C1006-50-F | Spin-Away Filters | 50 Pack | $62.30 | |
| W1001-4 | DNase/RNase-Free Water | 4 ml | $13.60 | |
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