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Quick-cfDNA Serum & Plasma Kit
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Cat # | Name | Size | Price | Quantity |
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Highlights
- High Processing Volume: Purify ≤ 10 ml of serum or plasma and elute with 35 µl.
- Highest Yields: Consistently purify > 30% more cfDNA.
- Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, etc.
Documents
Product Description
Technical Specifications
DNA Purity | High-quality DNA is ready for all sensitive downstream applications such as qPCR and Next-Generation sequencing. |
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DNA Recovery | Recover DNA ≥ 100bp (optimized for recovery of cell-free DNA). Yields can vary considerably among different individuals. Typically DNA recovery ranges from 1-100 ng/ml of plasma or serum. The yield varies depending on the sample source and the health of the donor. |
DNA Storage | Eluted DNA should be stored at ≤ -20ºC |
Processing volume | Plasma – Single centrifugation: up to 3 ml Plasma – Double centrifugation: up to 10 ml Serum – up to 10 ml Amniotic fluid – up to 1 m l Cerebrospinal fluid (CSF) – up to 1 ml Saliva – up to 1 ml Cell-free saliva – up to 5 ml |
Recovery Volume | ≥ 35 µl of DNA Elution Buffer or DNase free water. |
Required Equipment | Water bath or heat block (55ºC), microcentrifuge, vacuum/vacuum manifold or swinging bucket centrifuge. |
Sample Sources | Serum, plasma, amniotic fluid, and cerebrospinal fluid (CSF). |
Supplemental Info
Resources
Q1: Is the kit compatible to Streck tubes and PAXgene cfDNA tubes?
Yes, both Streck DNA and PAXgene tubes are compatible. First pellet down samples, remove the supernatant, and proceed with the protocol.
Q2: How to process samples stabilized in DNA/RNA Shield with the kit?
This kit is not compatible with samples stored in DNA/RNA Shield.
Cat # | Name | Size | Price | |
---|---|---|---|---|
D3004-4-4 | DNA Elution Buffer | 4 ml | $11.30 | |
D4076-1-32 | S&P 5X Digestion Buffer | 32 ml | $43.10 | |
D4076-2-170 | S&P DNA Binding Buffer | 170 ml | $185.80 | |
D4076-3-10 | S&P DNA Prep Buffer | 10 ml | $22.70 | |
D4076-4-12 | S&P DNA Wash Buffer (Concentrate) | 12 ml | $34.00 | |
C1001-50 | Collection Tubes | 50 Pack | $17.00 | |
C1049-25 | Zymo-Spin III-S Columns w/ LuerLock Caps and 15 ml Reservoir | 25 Pack | $133.70 | |
D3001-2-125 | Proteinase K w/ Storage Buffer Set | 125 mg | $257.20 | |
Flexibil[e] for input volumes [with] quick references.”
- E. L. (Cepheid)
“Easy to use and good yield.”
- X.Q (University of California, San Diego)
“Higher DNA concentration in the final eluate from the Zymo kit when compared to the Qiagen kit. Total yield, measured by reference genes, is higher for the Zymo kit when compared to the Qiagen kit.”
- T.R. (Aarhus University Hospital)
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