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    YeaStar Genomic DNA Kit


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    Cat # Name Size Price Quantity
    D2002 YeaStar Genomic DNA Kit 40 Preps $156.40
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    Highlights

    • Simple: Fast spin-column procedure yields pure yeast genomic DNA without using glass beads or phenol.
    • Versatile: Efficient DNA isolation from a broad spectrum of fungal species susceptible to Zymolyase.
    • High-Quality: Isolated genomic DNA is ready for Southern blotting, PCR, restriction enzyme digestions, etc.
    Description & Documents Specifications FAQ Components

    Documents

    Product Description

    The YeaStar Genomic DNA Kit is designed for reliable and efficient isolation of genomic DNA from a broad spectrum of fungal species, including Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The kit is based on highly efficient enzyme lysis and Zymo-Spin column technology. Each standard prep yields about 7 - 20 µg of DNA with a size distribution of 35 - 60 kb. The resulting genomic DNA can be used for direct analysis including Southern blotting, PCR, restriction endonuclease digestion, etc.

    Documents

    Technical Specifications

    Applicable For Isolated DNA is well suited for downstream applications such as Southern blotting, PCR, restriction endonuclease digestion, Next-Generation Sequencing and other sensitive applications.
    Elution Volume ≥ 60 µl TE or DNase-free Water
    Equipment Microcentrifuge, heat block/bath
    Processing Time 1.5 hours
    Processing Volume ≤ 1.5 ml of Culture (1-5 x 107 cells)
    Purity Typical A260/A280 & A260/A230 ≥ 1.8
    Sample Source Cell Culture (Colonies/Patches or Liquid Culture)
    Size Range Capable of recovering 35-60 kb sized fragments of genomic and mitochondrial DNA.
    Type Total DNA
    Yield ≤ 25 µg of total DNA

    Resources

    Protocol I and II are almost same, except that chloroform is used in Protocol I. Protocol I usually gives more recovery of DNA by 30-80% compared to Protocol II. Protocol II is chloroform-free. Chloroform is not provided.

    Any strains susceptible to Zymolyase, which includes the following fungal genera: Asbya, Kloekera, Candida, Kluyveromyces, Debaryomyces, Lipomyces, Eremothecium, Metschikowia, Endomyces, Pichia, Hansenula, Pullularia, Hanseniaspora, Saccharomyces, Saccaromycodes, Saccharomycopsis, Schizosaccahromyces, Torulopsis

    Zymolyase is very efficient, but here are some tips to maximize DNA yield. 1. Use fresh and early log phase cells, which are more susceptible to the lytic properties of Zymolyase. 2. Using less cells as input can improve overall lysis efficiency. 3. Zymolyase digestion time can also be increased to 2 – 16 hours.

    Cat # Name Size Price
    D2002-1 YD Digestion Buffer 4.8 ml $20.40
    D2002-2 YD Lysis Buffer 4.8 ml $35.10
    D4003-2-6 DNA Wash Buffer (Concentrate) 6 ml $11.30

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