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    Quick SARS-CoV-2 Multiplex Kit

    R3013 / R3013-1K / R3013-10K


    Quick SARS-CoV-2 Multiplex Kit

    Cat # Name Size
    R3013 Quick SARS-CoV-2 Multiplex Kit 100 rxns
    R3013-1K Quick SARS-CoV-2 Multiplex Kit 1,000 rxns
    R3013-10K Quick SARS-CoV-2 Multiplex Kit 10,000 rxns
    Contact for Pricing
    Documents
    Instructions for Use - CE-IVD: 
    Instructions for Use - RUO: 
    SDS (MSDS) EU US
    Kit Brochure: 
    KingFisher™ Script: 

    Highlights
    • High Sensitivity: Limit of Detection as low as 10 GEC/reaction (167 GEC/ml)
    • Specific: Detection of SARS-CoV-2 and Emerging Strains (including Omicron)
    • Rapid & Easy Setup: Ready-to-use Master Mix, just add sample.
    • Compatible with Automated and High-Throughput workflows.
    • CE-IVD Marked
    Description

    The Quick SARS-CoV-2 Multiplex Kit is a real-time reverse transcription PCR (rRT-PCR) test for the qualitative detection of RNA from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which is responsible for the coronavirus disease (COVID-19).

    The Quick SARS-CoV-2 Multiplex Kit targets a region of the viral nucleocapsid (N) gene and a host-specific target region (human RNase P gene) to assess sample quality. The kit also includes CV Positive Control that enable assay performance monitoring, and a No-Template Control to confirm the absence of contamination in the reagents.

    The Quick SARS-CoV-2 Multiplex Kit can be used on purified RNA samples isolated from upper respiratory and lower respiratory systems.

    The Quick SARS-CoV-2 Multiplex Kit has High Sensitivity, with a Limit of Detection as low as 10 viral genome equivalent copies per reaction, a fast turnaround time of less than 2 hours, and a simple workflow in which RNA is simply added to the Quick SARS-CoV-2 Multiplex Kit reagent and directly analyzed.

    Due to its simplicity, set-up up can be performed in an automation and it is compatible with high-throughput platforms. The kit is compatible with multiple Real-Time PCR instruments capable of detecting HEX/VIC and Quasar 670/Cy5 fluorophores and technical support is available for all steps of the setup process and data interpretation.

    Learn More

    In the United States:
    -This test has not been FDA cleared or approved and it is currently CE IVD marked.

    Compatibility Compatible with automated and high-throughput workflows.
    Equipment Required Real-Time PCR Instruments capable of detecting HEX/VIC and Quasar® 670/Cy5 fluorophores. Adjustments of the RT-PCR parameters may be necessary for instruments different than the CFX96 Touch from Bio-Rad.
    Input Quality Purified RNA free of enzymatic inhibitors.
    Processing Time ≤ 2 hours from set up to results.
    Reagents Complete and ready to use master mix.
    Registration Status CE-IVD Marked
    Sample Input Material Purified RNA from upper respiratory and lower respiratory systems.

    This test has not ben FDA cleared or approved. In the USA this test can be adopted in clinical diagnostics laboratories as an LDT according to local state guidelines. The test can be used for RUO/Informational purposes. The test is currently pending for CE IVD certification.

    This test detects specifically SARS-CoV-2 and cannot be used to detect other viruses or pathogens.

    Please refer to the Interpretation of Results, the Appendix, and the Troubleshooting Guide sections in our Quick SARS-CoV-2 Multiplex Kit protocol. If you still have problems with the result interpretation please contact our Technical Support team at (949) 679-1190 ext. 3 or e-mail us at tech@zymoresearch.com.

    Sometimes aberrant qPCR signal are observed. Please refer to the Interpretation of Results, the Appendix, and the Troubleshooting Guide sections in our Quick SARS-CoV-2 Multiplex Kit protocol. If you still have problems with the result interpretation please contact our Technical Support team at (949) 679-1190 ext. 3 or e-mail us at tech@zymoresearch.com.

    This may indicate incorrect plate set-up or a contamination in the No-Template Control or in the 2X CV Mix. We recommend repeating the test, and if the problem persists to use a new aliquot of reagents.

    If using RT-PCR instruments different than the CFX96 Touch from Bio-Rad, this problem can be solved by adjusting the RT-PCR parameters (e.g. baseline threshold, temperature ramp, ...).
    This problem may also indicate incorrect plate set up of the compromise of Quick SARS-CoV-2 reagents.
    Please contact our Technical Support team at (949) 679-1190 ext. 3 or e-mail us at tech@zymoresearch.com

    If the host target didn’t amplify or shows CT values above 40, your RNA extraction and/or RT-PCR reaction may have been incorrectly performed. We suggest repeating RNA extraction and the RT-PCR reaction.

    Late signal for SARS-CoV-2 viral targets may be indicative of a low viral load in your sample. We suggest repeating the RT-PCR to confirm results.

    With a limit of detection(LoD) as low as 10 GEC/reaction the Quick SARS-CoV-2 Multiplex Kit outperforms most of the SARS-CoV-2 molecular tests present on the market.
    In addition, the detection of a host target (human Rnase P) allow the identification of insufficient samples, which other tests may classify as negative; this further reduce the chances of the false negative results.

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