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Highlights
- Complete bisulfite conversion of DNA in less than 3 hours.
- Desulphonation and recovery of bisulfite-treated DNA with a spin column.
- Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
Highlights
- Complete bisulfite conversion of DNA in less than 3 hours.
- Desulphonation and recovery of bisulfite-treated DNA with a spin column.
- Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
| Cat # | Name | Size | Price | Quantity |
|---|
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| D5002-4 | M-Wash Buffer | 24 ml | $31.80 | |
| D5001-4 | M-Wash Buffer | 6 ml | $12.80 | |
| D5001-5 | M-Desulphonation Buffer | 10 ml | $19.10 | |
| D5002-5 | M-Desulphonation Buffer | 40 ml | $53.50 | |
| D5005-2 | M-Dilution Buffer-Gold | 1.5 ml | $12.80 | |
| D5006-2 | M-Dilution Buffer-Gold | 7 ml | $12.80 | |
| D5005-3 | M-Binding Buffer | 30 ml | $25.40 | |
| D5002-6 | M-Elution Buffer | 4 ml | $12.80 | |
| D5001-6 | M-Elution Buffer | 1 ml | $12.80 | |
| D5006-3 | M-Binding Buffer | 125 ml | $73.90 | |
| D5005-6 | M-Dissolving Buffer | 500 µl | $12.80 | |
| D5006-6 | M-Dissolving Buffer | 1.2 ml | $19.10 | |
| C1001-50 | Collection Tubes | 50 Pack | $17.50 | |
| C1004-50 | Zymo-Spin IC Columns | 50 Pack | $61.80 | |
| D5001-1 | CT Conversion Reagent | 1 tube for 10 Conversions | $12.80 | |
Description
Performance
Technical Specifications
| Applications | Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. |
|---|---|
| Conversion | >99% |
| Elution Volume | ≥ 10 µl |
| Equipment | Microcentrifuge and thermocycler with heated lid |
| Input | 500 pg - 2 µg of DNA. |
| Processing Time | 3 hours |
| Recovery | >75% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
Resources
Documents
FAQ
Leaving the desulphonation buffer on the column longer than recommended will cause more degradation and subsequently result in lower yields.
Bisulfite conversion will work regardless of context, so the kits are compatible with genomic DNA derived from plants and other species with high non-CpG methylation levels.
ZymoTaq DNA Polymerase has been specifically designed for use in bisulfite amplification reactions. ZymoTaq is available as a stand-alone polymerase (E2001/E2002), PreMix (E2003/E2004), and qPCR PreMix (E2054/E2055).
> 50 bp.
For best results, keep the method of quantification consistent before and after bisulfite treatment:
- If quantifying with a NanoDrop, use dsDNA settings (50 μg/ml for Ab260 = 1.0) before treatment and use RNA settings (40 μg/ml for Ab260 = 1.0) after treatment.
- If quantifying with Qubit, use a dsDNA assay before treatment and use a ssDNA assay after treatment.
Following bisulfite treatment, DNA will be single stranded with limited non-specific base pairing at room temperature. To visualize, run the converted DNA on an agarose gel then chill the gel on ice or in an ice bath for 30 minutes. This will force enough base-pairing to allow intercalation of the ethidium bromide for the DNA to be visible. If using a Bioanalyzer or TapeStation instrument, use RNA kits and reagents to visualize the converted DNA.
Poor conversion efficiency and low yields can be due to a variety of different experiment-specific conditions. Please contact Technical Support to discuss your specific experimental conditions and further troubleshoot with a product specialist.
Converted DNA eluted in M-Elution Buffer can generally be stored at -20°C for 1-3 months. If longer term storage is necessary, we recommend storing at or below -70°C if possible. Bisulfite converted DNA is less stable than dsDNA; for best results, minimize freeze-thawing of converted DNA and use as soon as possible for downstream analysis.
Reviews
"It makes gene specific DNA methylation analysis very simple and anyone with modest technical background can easily perform the experiment using this kit."
Murali B.
Centre for DNA Fingerprinting and Diagnostics
"This protocol allows for bisulfite conversion in about 3 hours. This fast conversion is particularly relevant to our experiments, which allows us to PCR amplify and clone the products in 1 day, saving time without loss of quality."
Joseph M.
Sanford-Burnham Medical Research Institute
Citations
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