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    Quick-RNA Fungal/Bacterial Miniprep Kit


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    Cat # Name Size Price Quantity
    R2014 Quick-RNA Fungal/Bacterial Miniprep Kit 50 preps $288.90
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    Highlights

    • High Quality: Extract intact high quality total RNA (including small/micro RNAs) from Gram-negative/postive bacteria, yeast, and fungi
    • Homogenization: Ultra-high density BashingBead technology for complete disruption and sample lysis.
    • Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.
    Description & Documents Specifications Components

    Documents

    Product Description

    The Quick-RNA Fungal/Bacterial Microprep Kit is designed for the simple and rapid isolation of RNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the RNA that is ideal for downstream molecular-based applications including Next-Gen sequencing, RT-PCR, microarray, hybridization, etc.

    Documents

    Technical Specifications

    Applicable For For all sensitive downstream applications, such as Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc.
    Elution Volume 25 µl
    Equipment Microcentrifuge, vortex, cell disrupter/pulverizer
    Processing Time ≤ 10 minutes
    Processing Volume 50-100 mg (wet weight) fungi or bacteria. 109 bacterial cells and 108 yeast cells
    Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc.
    A260/A280, A260/A230: > 1.8.
    Sample Source Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled.
    Size Range Total RNA ≥ 17 nt
    Type Total RNA
    Yield Miniprep
    50 µg RNA (binding capacity)
    ≥ 25 µl (elution volume)

    Resources

    Cat # Name Size Price
    W1001-1 DNase/RNase-Free Water 1 ml $11.30
    C1006-50-G Zymo-Spin IIICG Columns 50 Pack $62.30
    C1001-50 Collection Tubes 50 Pack $17.00
    R1060-2-25 RNA Prep Buffer 25 ml $45.30
    R1003-3-24 RNA Wash Buffer 24 ml $66.80
    R1060-1-50 RNA Lysis Buffer 50 ml $89.50
    R1060-1-100 RNA Lysis Buffer 100 ml $171.10
    S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes $114.40
    C1078-50 Zymo-Spin IICR Columns 50 Pack $62.30

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